Cellomics and Toxicogenomics Research Core Laboratory

Director: Tchounwou, Paul

Summary:

"The Cellomics and Toxicogenomics Research Laboratory (CTRL) has been established to provide RCMI investigators and other JSU faculty and students with state-of-the art instrumentation that would allow them to perform laser-based flow cytometric analysis and cell sorting, as well as microarray analysis of gene expression in connection to human diseases. Hence, the application of flow cytometry and modern genomic DNA microarray-based technology is providing an excellent opportunity to CTRL users to examine alterations in gene expression patterns associated with environmental exposure, as well as to study the molecular mechanisms and biochemical effects associated with cellular responses to toxic exposures."(http://www.jsums.edu/cset/rcmi/ctrl.htm)

Affiliations:

• Department of Biology, Jackson State University

People:

• Director: Tchounwou, Paul
  

Member: Yedjou, Clement G.

Resources:

Instruments

• Affymetrix Chip Scanner ( Array scanner )
  

• Affymetrix GeneChip Fluidics Station 400 ( Fluidics station )
  

• BD FACSCalibur flow cytometer ( Flow cytometer )
  

• BD FACSVantage flow cytometer ( Flow cytometer )
  

• iCycler PCR ( PCR instrument )
  

• Leica Confocal Microscope ( Confocal microscope )
  

Protocols

• Apoptosis assay protocol (Annexin V - FITC) ( Protocol )
  

• Caspase apoptosis assay protocol ( Protocol )
  

• Cell morphology-based apoptosis assay protocol ( Protocol )
  

Reagents

• anti-Active Caspase-3-PE ( Polyclonal antibody reagent )
  

• BD Biosciences Annexin V FITC ( Reagent )
  

Services

• Cell and genomics instrument access ( Access service )
  

  Provides state-of-the art instrumentation that allows laser-based flow cytometric analysis and cell sorting, as well as microarray analysis of gene expression in connection to human diseases.

• Flow cytometry and microarray training service ( Training service )
  

  Provides training on laser-based flow cytometric analysis and cell sorting, as well as microarray analysis of gene expression in connection to human diseases.

Web Links:

• http://www.jsums.edu/cset/rcmi/ctrl.htm
• http://www.jsums.edu/cset/rcmi/index.htm